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Downregulated calmodulin expression contributes to endothelial cell impairment in diabetes

   
@article{APS10941,
	author = {Tian-tian Liu and Huan-huan Xu and Ze-juan Liu and He-ping Zhang and Hai-tao Zhou and Zhi-xiang Zhu and Zhi-qiang Wang and Jing-yi Xue and Qiang Li and Yi Ma and Hong-jie You and Da-li Luo},
	title = {Downregulated calmodulin expression contributes to endothelial cell impairment in diabetes},
	journal = {Acta Pharmacologica Sinica},
	volume = {44},
	number = {12},
	year = {2023},
	keywords = {},
	abstract = {Endothelial dysfunction, a central hallmark of cardiovascular pathogenesis in diabetes mellitus, is characterized by impaired endothelial nitric oxide synthase (eNOS) and NO bioavailability. However, the underlying mechanisms remain unclear. Here in this study, we aimed to identify the role of calmodulin (CaM) in diabetic eNOS dysfunction. Human umbilical vein endothelial cells and murine endothelial progenitor cells (EPCs) treated with high glucose (HG) exhibited downregulated CaM mRNA/protein and vascular endothelial growth factor (VEGF) expression with impeded eNOS phosphorylation and cell migration/tube formation. These perturbations were reduplicated in CALM1-knockdown cells but prevented in CALM1-overexpressing cells. EPCs from type 2 diabetes animals behaved similarly to HG-treated normal EPCs, which could be rescued by CALM1-gene transduction. Consistently, diabetic animals displayed impaired eNOS phosphorylation, endothelium-dependent dilation, and CaM expression in the aorta, as well as deficient physical interaction of CaM and eNOS in the gastrocnemius. Local CALM1 gene delivery into a diabetic mouse ischemic hindlimb improved the blunted limb blood perfusion and gastrocnemius angiogenesis, and foot injuries. Diabetic patients showed insufficient foot microvascular autoregulation, eNOS phosphorylation, and NO production with downregulated CaM expression in the arterial endothelium, and abnormal CALM1 transcription in genome-wide sequencing analysis. Therefore, our findings demonstrated that downregulated CaM expression is responsible for endothelium dysfunction and angiogenesis impairment in diabetes, and provided a novel mechanism and target to protect against diabetic endothelial injury.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/10941}
}