@article{APS10849,
author = {Yang Li and Qiao Yin and Qi Li and An-ran Huo and Ting-ting Shen and Jia-qian Cao and Chun-feng Liu and Tong Liu and Wei-feng Luo and Qi-fei Cong},
title = {Botulinum neurotoxin A ameliorates depressive-like behavior in a reserpine-induced Parkinson’s disease mouse model via suppressing hippocampal microglial engulfment and neuroinflammation},
journal = {Acta Pharmacologica Sinica},
volume = {44},
number = {7},
year = {2023},
keywords = {},
abstract = {Depression is one of the common non-motor symptoms of Parkinson’s disease (PD). In the clinic, botulinum neurotoxin A (BoNT/A) has been used to treat depression. In this study, we investigated the mechanisms underlying the anti-depressive effect of BoNT/A in a PD mouse model. Mice were administered reserpine (3 μg/mL in the drinking water) for 10 weeks. From the 10th week, BoNT/A (10 U·kg−1·d−1) was injected into the cheek for 3 consecutive days. We showed that chronic administration of reserpine produced the behavioral phenotypes of depression and neurochemical changes in the substantia nigra pars compacta (SNpc) and striatum. BoNT/A treatment significantly ameliorated the depressive-like behaviors, but did not improve TH activity in SNpc of reserpine- treated mice. We demonstrated that BoNT/A treatment reversed reserpine-induced complement and microglia activation in the hippocampal CA1 region. Furthermore, BoNT/A treatment significantly attenuated the microglial engulfment of presynaptic synapses, thus ameliorating the apparent synapse and spine loss in the hippocampus in the reserpine-treated mice. Moreover, BoNT/A treatment suppressed microglia-mediated expression of pro-inflammatory cytokines TNF-α and IL-1β in reserpine-treated mice. In addition, we showed that BoNT/A (0.1 U/mL) ameliorated reserpine-induced complement and microglia activation in mouse BV2 microglial cells in vitro. We conclude that BoNT/A ameliorates depressive-like behavior in a reserpine-induced PD mouse model through reversing the synapse loss mediated by classical complement induced-microglial engulfment as well as
alleviating microglia-mediated proinflammatory responses.
● Botulinum neurotoxin A ameliorates reserpine-induced depressive behaviors.
● Complement activation is involved in the reserpine-induced mouse model.
● Botulinum neurotoxin A reverses spine loss and reduced synapse density.
● Botulinum neurotoxin A inhibits complement activation and microglial engulfment.
● Botulinum neurotoxin A alleviates reserpine-induced neuroinflammation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/10849}
}