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Comparative strategies for stem cell biodistribution in a preclinical study

   
@article{APS10129,
	author = {Fang Wang and Zhe Wang and Fen Wang and Kelly Dong and Jing Zhang and Yun-juan Sun and Chun-feng Liu and Mei-jie Xing and Xue Cheng and Su Wei and Jia-wei Zheng and Xiong-fei Zhao and Xiao-ming Wang and Jie Fu and Hai-feng Song},
	title = {Comparative strategies for stem cell biodistribution in a preclinical study},
	journal = {Acta Pharmacologica Sinica},
	volume = {41},
	number = {4},
	year = {2020},
	keywords = {},
	abstract = {Stem cell therapy represents the potential alternative effective strategy for some diseases that lack effective treatment currently. Correspondingly, it is crucial to establish high-sensitive and reliable quantification assay for tracing exogenous cell migration. In the present study, we first used both bioluminescence imaging (BLI) indirect labeling (human norepinephrine transporter-luciferase reporter system) and 89zirconium (89Zr)-hNSCs direct labeling combined with positron emission tomography/computer tomography (PET/CT) system for tracking human neural stem cells (hNSCs) migration into the brain via nasal administration in preclinical study. But the above two methods failed to give the biodistribution profile due to their low sensitivity. Considering its superior sensitivity and absolute quantitation capability, we developed and validated the droplet digital PCR (ddPCR) targeting species-specific gene in frozen and paraffin sections, slices, and whole blood with the sensitivity of 100–200 hNSCs. Accurate and high throughput quantification could be performed using ddPCR with the coefficient of variation (CVs) of lower quality control (LQC) below 30%. In combination with immunohistochemistry and ddPCR, we confirmed the migration of hNSCs into the brain via nasal administration, which supported the efficacy of hNSCs in MPTP-treated mice, an animal model of Parkinson’s disease. In conclusion, the present study is the first to report the application of ddPCR in the pharmacokinetics profile description of tracking of hNSCs in preclinical studies.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/10129}
}