Effects of protein kinase C activator and inhibitor on release of tumor necrosisfactor from mouse peritoneal macrophages

The effects of protein kinase C (PKC) activator 1-O-tetradecanoylphorbol-13-acetate (TPA) and inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7) and quercetin were studied on release of tumor necrosis factor (TNF) from mouse peritoneal macrophages primed with Propionibacterium acnes (PA). The results showed that TPA (1-100 ng.ml-1) and lipopolysaccharides (LPS) (1-100 ng.ml-1) induced the release of TNF from PA-primed mouse peritoneal macrophages in dose- and time-dependent manners in vitro, and the effects of TPA and LPS were inhibited by H-7 (12.5-100 mumol.L-1) or quercetin (6.25-25 mumol.L-1) in a dose-dependent manner. After ip H-7 (50 mg.kg-1), LPS-induced release of TNF in vivo decreased significantly. These results suggest that PKC may play a critical role in release of TNF from PA-primed macrophages.