Effects of changrolin on potassium currents in guinea pig and rabbit single heart cells.
Abstract
AIM:
To elucidate whether or not changrolin (CRL) modifies the potassium currents (ITO, IK, and IKl) in myocardial cells.
METHODS:
A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IKl in single cells isolated from guinea pig and rabbit hearts.
RESULTS:
At a clinically relevant concentration, CRL 50 mumol.L-1 inhibited the transient outward current (ITO) by 17.7% +/- 2.4% (n = 8) in rabbit atrial cells. The voltage-dependence of steady-state inactivation of ITO was not affected by CRL. This concentration of CRL did not influence the time-independent inward rectifier or the delayed rectifier K+ currents (IKl and IK, respectively) in rabbit and guinea pig ventricular cells.
CONCLUSION:
CRL inhibited ITO, but not IK nor IKl.
Keywords:
To elucidate whether or not changrolin (CRL) modifies the potassium currents (ITO, IK, and IKl) in myocardial cells.
METHODS:
A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IKl in single cells isolated from guinea pig and rabbit hearts.
RESULTS:
At a clinically relevant concentration, CRL 50 mumol.L-1 inhibited the transient outward current (ITO) by 17.7% +/- 2.4% (n = 8) in rabbit atrial cells. The voltage-dependence of steady-state inactivation of ITO was not affected by CRL. This concentration of CRL did not influence the time-independent inward rectifier or the delayed rectifier K+ currents (IKl and IK, respectively) in rabbit and guinea pig ventricular cells.
CONCLUSION:
CRL inhibited ITO, but not IK nor IKl.