Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China.
Abstract
AIM:
To study the effect of dopamine receptor antagonists on anoxia-induced inhibition of Ca(2+)-calmodulin dependent protein kinase II (CCDPK II) activity in rat hippocampus and striatum.
METHODS:
Using the rat hippocampal and striatal slices under 95% N2 + 5% CO2, the activity of CCDPK II was examined by 32P-incorporation.
RESULTS:
Under anoxia for 30 min, the CCDPK II activity decreased to 29.2% and 27.0% of the control in rat hippocampal and striatal slices, respectively. Preincubation with Sch-23390 (a specific D1-like dopamine receptor antagonist), or domperidone (a specific D2-like dopamine receptor antagonist), resulted in a concentration-dependent attenuation of the anoxia-induced inhibition of CCDPK II activity which was preserved up to about 60%.
CONCLUSION:
Dopamine receptor stimulation is involved in anoxia-induced inhibition of CCDPK II activity in rat hippocampus and striatum.
Keywords:
To study the effect of dopamine receptor antagonists on anoxia-induced inhibition of Ca(2+)-calmodulin dependent protein kinase II (CCDPK II) activity in rat hippocampus and striatum.
METHODS:
Using the rat hippocampal and striatal slices under 95% N2 + 5% CO2, the activity of CCDPK II was examined by 32P-incorporation.
RESULTS:
Under anoxia for 30 min, the CCDPK II activity decreased to 29.2% and 27.0% of the control in rat hippocampal and striatal slices, respectively. Preincubation with Sch-23390 (a specific D1-like dopamine receptor antagonist), or domperidone (a specific D2-like dopamine receptor antagonist), resulted in a concentration-dependent attenuation of the anoxia-induced inhibition of CCDPK II activity which was preserved up to about 60%.
CONCLUSION:
Dopamine receptor stimulation is involved in anoxia-induced inhibition of CCDPK II activity in rat hippocampus and striatum.