Effect of nitric oxide on electric and mechanical activities of gastric antral circular muscles in guinea pigs
Abstract
"AIM:
To study the effect of exogenous nitric oxide (NO) on electric and mechanical activities of gastric antral circular muscle in guinea pigs in vitro.
METHODS:
Mechanical and electric activities of gastric antral circular muscle in guinea pigs were recorded simultaneously.
RESULTS:
Sodium nitroprusside (SNP, 0.5 mumol.L-1), an NO donor, inhibited the frequency and amplitude of fast wave and spontaneous contraction of the strips (P < 0.01). SNP-induced inhibition was not blocked by tetrodotoxin, atropine, phentolamine, and propranolol (P > 0.05), but diminished by methylene blue (P < 0.01) and oxyhemoglobin (P < 0.01).
CONCLUSION:
Exogenous NO inhibits gastric antral myoelectric and mechanical activities in guinea pigs. The inhibitions are produced by NO acting on extracellular membrane and enhancing the level of cGMP."
Keywords:
To study the effect of exogenous nitric oxide (NO) on electric and mechanical activities of gastric antral circular muscle in guinea pigs in vitro.
METHODS:
Mechanical and electric activities of gastric antral circular muscle in guinea pigs were recorded simultaneously.
RESULTS:
Sodium nitroprusside (SNP, 0.5 mumol.L-1), an NO donor, inhibited the frequency and amplitude of fast wave and spontaneous contraction of the strips (P < 0.01). SNP-induced inhibition was not blocked by tetrodotoxin, atropine, phentolamine, and propranolol (P > 0.05), but diminished by methylene blue (P < 0.01) and oxyhemoglobin (P < 0.01).
CONCLUSION:
Exogenous NO inhibits gastric antral myoelectric and mechanical activities in guinea pigs. The inhibitions are produced by NO acting on extracellular membrane and enhancing the level of cGMP."