Dauricine inhibits redistribution of platelet membrane glycoprotein IV and release of intracellular alpha-granule thrombospondin induced by thrombin
Abstract
AIM:
To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein IV (GPIV) and release of intracellular alpha-granule thrombospondin (TSP) on platelet activation.
METHODS:
Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin.
RESULTS:
Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPIV and TSP (r = 0.511, P < 0.01). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent.
CONCLUSION:
Dau inhibited redistribution of GPIV and release of intracellular alpha-granule thrombospondin induced by thrombin.
Keywords:
To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein IV (GPIV) and release of intracellular alpha-granule thrombospondin (TSP) on platelet activation.
METHODS:
Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin.
RESULTS:
Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPIV and TSP (r = 0.511, P < 0.01). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent.
CONCLUSION:
Dau inhibited redistribution of GPIV and release of intracellular alpha-granule thrombospondin induced by thrombin.