Protective effect of ginsenoside Rg1 against MPTP-induced apoptosis in mouse substantia nigra neurons.
Abstract
AIM: To explore the possible mechanism of the ginsenoside Rg1 in protecting
substantia nigra neurons from 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine
(MPTP)-induced apoptosis in C57BL mice.
METHODS: C57BL male mice were given with MPTP to prepare Parkinson's disease
mouse model. Different doses of Rg1 (2.5, 5.0, and 10.0 mg/kg, respectively) were
given 3 d prior to MPTP in the pretreatment groups. Nissl staining, TH
immunostaining, and TUNEL labeling were used to observe the damage and apoptosis
of nigral neurons. The immunohistochemistry assay was used to detect the protein
levels of Bcl-2, Bcl-xl, Bax, inducible nitric oxide synthase (iNOS), neuronal
NOS (nNOS), and cleaved caspase-3.
RESULTS: Compared with MPTP model group, pretreatment with Rg1 (5.0 and 10.0
mg/kg) was shown to increase the Nissl staining neurons and TH-positive neurons
(P<0.01), and to decrease the TUNEL-positive neurons in the substantia nigra zona
compacta (P<0.01). Moreover, Rg1 elevated the levels of cleaved caspase-3, Bax,
and iNOS, but reduced the levels of Bcl-2 and Bcl-xl (P<0.01).
CONCLUSION: Rg1 has protective effect against MPTP-induced apoptosis and this
effect may be attributed to enhancing Bcl-2 and Bcl-xl expression, reducing Bax
and iNOS expression, and inhibiting activation of caspase-3.
Keywords:
substantia nigra neurons from 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine
(MPTP)-induced apoptosis in C57BL mice.
METHODS: C57BL male mice were given with MPTP to prepare Parkinson's disease
mouse model. Different doses of Rg1 (2.5, 5.0, and 10.0 mg/kg, respectively) were
given 3 d prior to MPTP in the pretreatment groups. Nissl staining, TH
immunostaining, and TUNEL labeling were used to observe the damage and apoptosis
of nigral neurons. The immunohistochemistry assay was used to detect the protein
levels of Bcl-2, Bcl-xl, Bax, inducible nitric oxide synthase (iNOS), neuronal
NOS (nNOS), and cleaved caspase-3.
RESULTS: Compared with MPTP model group, pretreatment with Rg1 (5.0 and 10.0
mg/kg) was shown to increase the Nissl staining neurons and TH-positive neurons
(P<0.01), and to decrease the TUNEL-positive neurons in the substantia nigra zona
compacta (P<0.01). Moreover, Rg1 elevated the levels of cleaved caspase-3, Bax,
and iNOS, but reduced the levels of Bcl-2 and Bcl-xl (P<0.01).
CONCLUSION: Rg1 has protective effect against MPTP-induced apoptosis and this
effect may be attributed to enhancing Bcl-2 and Bcl-xl expression, reducing Bax
and iNOS expression, and inhibiting activation of caspase-3.