Effects of dl-praeruptorin A on interleukin-6 level and Fas, bax, bcl-2 protein expression in ischemia-reperfusion myocardium.
Abstract
AIM: To investigate the effects of dl-praeruptorin (Pd-Ia) on interleukin-6
(IL-6) level and apoptosis-related protein expression in ischemia-reperfusion
myocardium.
METHODS: Left anterior descending coronary artery was subjected to 30 min
ischemia followed by 120 min reperfusion in open-chest anesthetized rats. Serum
IL-6 level was measured by radioimmunoassay. Apoptosis-related protein Fas, bax,
and bcl-2 expression was detected by immunohistochemistry and computer image
analysis system. Infiltration of neutrophils was observed using Hematoxylin-Eosin
staining under optical microscope.
RESULTS: Pd-Ia 2.0 mg/kg iv lowered serum IL-6 level and Fas, bax, bcl-2
expression under conditions with hypotension and without changes on heart rate,
but increased the ratio of bcl-2/bax. There existed a close linearity and
positive correlation between IL-6 level and Fas, bax, bcl-2 expression. Whereas,
the infiltration of neutrophils was mild.
CONCLUSION: Pd-Ia elicits a novel target in the therapeutic prevention of
postischemic cardiomyocyte death. The reason might be associated with modulating
the expression of some immediate-early genes including IL-6, Fas, bax, and bcl-2
in ischemia-reperfusion myocardium.
Keywords:
(IL-6) level and apoptosis-related protein expression in ischemia-reperfusion
myocardium.
METHODS: Left anterior descending coronary artery was subjected to 30 min
ischemia followed by 120 min reperfusion in open-chest anesthetized rats. Serum
IL-6 level was measured by radioimmunoassay. Apoptosis-related protein Fas, bax,
and bcl-2 expression was detected by immunohistochemistry and computer image
analysis system. Infiltration of neutrophils was observed using Hematoxylin-Eosin
staining under optical microscope.
RESULTS: Pd-Ia 2.0 mg/kg iv lowered serum IL-6 level and Fas, bax, bcl-2
expression under conditions with hypotension and without changes on heart rate,
but increased the ratio of bcl-2/bax. There existed a close linearity and
positive correlation between IL-6 level and Fas, bax, bcl-2 expression. Whereas,
the infiltration of neutrophils was mild.
CONCLUSION: Pd-Ia elicits a novel target in the therapeutic prevention of
postischemic cardiomyocyte death. The reason might be associated with modulating
the expression of some immediate-early genes including IL-6, Fas, bax, and bcl-2
in ischemia-reperfusion myocardium.