Differential effects of allopregnanolone and GABA on kainate-induced lactate dehydrogenase release in cultured rat cerebral cortical cells.
Abstract
AIM: To exam the effects of allopregnanolone and gamma-amino-butyric acid (GABA)
on the excitotoxicity.
METHODS: The excitotoxicity was evoked by kainate (KA) in the primary culture of
rat cerebral cortical cells. Effect of allopregnanolone or GABA on the
excitotoxicity was examined by the measurement of lactate dehydrogenase (LDH)
activity released in the culture medium.
RESULTS: Either acute (3 h) or chronic (24 h) treatment with KA (0.01-1 mmol/L)
produced a concentration-dependent increase in LDH activity released. The EC50
values were (0.16+/-0.03) mmol/L and (0.257+/-0.015) mmol/L, respectively. Acute
treatment with allopregnanolone (10-1000 nmol/L) for 3 h did not significantly
affect the 0.2 mmol/L KA-induced LDH activity. On the other hand, chronic
treatment with allopregnanolone (10-1000 nmol/L) for 24 h, produced inhibition on
the KA-induced LDH activity in a concentration-dependent manner. The EC50 value
was (436+/-19) nmol/L. Acute treatment with GABA (0.1-100 micromol/L) exacerbate
the 0.2 mmol/L KA-induced LDH activity in a concentration-dependent manner, with
an EC50 value of (2.7+/-1.0) micromol/L; while chronic treatment with GABA had no
significant effect.
CONCLUSION: There were differential patterns between the effects of
allopregnanolone and GABA on the KA-induced excitotoxicity.
Keywords:
on the excitotoxicity.
METHODS: The excitotoxicity was evoked by kainate (KA) in the primary culture of
rat cerebral cortical cells. Effect of allopregnanolone or GABA on the
excitotoxicity was examined by the measurement of lactate dehydrogenase (LDH)
activity released in the culture medium.
RESULTS: Either acute (3 h) or chronic (24 h) treatment with KA (0.01-1 mmol/L)
produced a concentration-dependent increase in LDH activity released. The EC50
values were (0.16+/-0.03) mmol/L and (0.257+/-0.015) mmol/L, respectively. Acute
treatment with allopregnanolone (10-1000 nmol/L) for 3 h did not significantly
affect the 0.2 mmol/L KA-induced LDH activity. On the other hand, chronic
treatment with allopregnanolone (10-1000 nmol/L) for 24 h, produced inhibition on
the KA-induced LDH activity in a concentration-dependent manner. The EC50 value
was (436+/-19) nmol/L. Acute treatment with GABA (0.1-100 micromol/L) exacerbate
the 0.2 mmol/L KA-induced LDH activity in a concentration-dependent manner, with
an EC50 value of (2.7+/-1.0) micromol/L; while chronic treatment with GABA had no
significant effect.
CONCLUSION: There were differential patterns between the effects of
allopregnanolone and GABA on the KA-induced excitotoxicity.