IL-10 and trichosanthin inhibited surface molecule expression of antigen processing cells and T-cell proliferation
Abstract
AIM:
To study immunoinhibitory effects and preliminary mechanism of IL-10 and trichosanthin.
METHODS:
Surface molecule expression on antigen processing cells (APC) was stained with fluorescence and analyzed by FACScan. B7-1 mRNA expression was detected with nested RT-PCR.
RESULTS:
IL-10 2 mg.L-1 and trichosanthin 10 mg.L-1 inhibited B7-1 molecule expression. By contrast, they had not the same effects on ICAM-1. IL-10 and trichosanthin down-regulated LFA-1 expression, but had no regulatory effect on CD40. IL-10 and trichosanthin dramatically inhibited T-cell proliferation and IL-2 production. B7-1 mRNA expression was undetectable in APC treated with IL-10 and trichosanthin.
CONCLUSION:
IL-10 and trichosanthin inhibit surface molecule expression on APC. They exert multiple immunoinhibitory effects.
Keywords:
To study immunoinhibitory effects and preliminary mechanism of IL-10 and trichosanthin.
METHODS:
Surface molecule expression on antigen processing cells (APC) was stained with fluorescence and analyzed by FACScan. B7-1 mRNA expression was detected with nested RT-PCR.
RESULTS:
IL-10 2 mg.L-1 and trichosanthin 10 mg.L-1 inhibited B7-1 molecule expression. By contrast, they had not the same effects on ICAM-1. IL-10 and trichosanthin down-regulated LFA-1 expression, but had no regulatory effect on CD40. IL-10 and trichosanthin dramatically inhibited T-cell proliferation and IL-2 production. B7-1 mRNA expression was undetectable in APC treated with IL-10 and trichosanthin.
CONCLUSION:
IL-10 and trichosanthin inhibit surface molecule expression on APC. They exert multiple immunoinhibitory effects.