Effect of rhynchophylline on apoptosis induced by dopamine in NT2 cells.
Abstract
AIM: To study the effect of rhynchophylline (Rhy) on the apoptosis induced by
dopamine (DA) in NT2 cell line.
METHODS: Release of lactate dehydrogenase (LDH) from cultured cells was
determined by enzyme-linked immunosorbant assay; apoptotic NT2 neurons by
terminal de oxynucleotidyl transferase(TdT)-mediated dUTP nick end labeling
method; DNA fragmentation by agarose gel electrophoresis; Bcl-2 protein
expression by Western blotting.
RESULTS: DA 100 - 500 micromol/L increased release of LDH, number of apoptotic NT
2 neurons, and DNA fragmentation (P < 0.01). NT2 cells survival rate was
decreased. Rhy 5 and 50 micromol/L reduced the percentage of apoptosis after
treated with DA 100 micromol/L for 6 h in both with and without bcl-2 gene
neurons (P < 0.05, P < 0.01, respectively). The expression of Bcl-2 protein was
not affected by Rhy.
CONCLUSION: Rhy protected NT2 neurons from the damage induced by DA.
Keywords:
dopamine (DA) in NT2 cell line.
METHODS: Release of lactate dehydrogenase (LDH) from cultured cells was
determined by enzyme-linked immunosorbant assay; apoptotic NT2 neurons by
terminal de oxynucleotidyl transferase(TdT)-mediated dUTP nick end labeling
method; DNA fragmentation by agarose gel electrophoresis; Bcl-2 protein
expression by Western blotting.
RESULTS: DA 100 - 500 micromol/L increased release of LDH, number of apoptotic NT
2 neurons, and DNA fragmentation (P < 0.01). NT2 cells survival rate was
decreased. Rhy 5 and 50 micromol/L reduced the percentage of apoptosis after
treated with DA 100 micromol/L for 6 h in both with and without bcl-2 gene
neurons (P < 0.05, P < 0.01, respectively). The expression of Bcl-2 protein was
not affected by Rhy.
CONCLUSION: Rhy protected NT2 neurons from the damage induced by DA.