Efflux transport of [3H]GABA across blood-brain barrier after cerebral ischemia-reperfusion in rats
Abstract
AIM:
To study whether the efflux transport of [3H]GABA across the blood-brain barrier (BBB) would be enhanced after cerebral ischemia-reperfusion.
METHODS:
Brain efflux index (BEI) of [3H]GABA was determined in ischemic-reperfused rats after [3H]GABA or [3H]GABA combined with unlabeled GABA or probenecid (Pro) was microinjected into the parietal cortex area 2 (Par 2), and brain uptake of Evans blue (EB) was assessed after i.v. EB.
RESULTS:
BEI in rats subjected to 10-min ischemia and 30-min, 2-h, 6-h, or 24-h reperfusion were 67%, 83%, 92%, and 87%, respectively, which were higher than that in control (58%). The brain uptake of EB was also considerably increased. Unlabeled GABA or Pro obviously decreased BEI in normal or 6-h reperfused rats, but GABA had no obvious effect on that in 5-min reperfused rats.
CONCLUSION:
The efflux transport of [3H]GABA was markedly enhanced after cerebral ischemia-reperfusion in rats.
Keywords:
To study whether the efflux transport of [3H]GABA across the blood-brain barrier (BBB) would be enhanced after cerebral ischemia-reperfusion.
METHODS:
Brain efflux index (BEI) of [3H]GABA was determined in ischemic-reperfused rats after [3H]GABA or [3H]GABA combined with unlabeled GABA or probenecid (Pro) was microinjected into the parietal cortex area 2 (Par 2), and brain uptake of Evans blue (EB) was assessed after i.v. EB.
RESULTS:
BEI in rats subjected to 10-min ischemia and 30-min, 2-h, 6-h, or 24-h reperfusion were 67%, 83%, 92%, and 87%, respectively, which were higher than that in control (58%). The brain uptake of EB was also considerably increased. Unlabeled GABA or Pro obviously decreased BEI in normal or 6-h reperfused rats, but GABA had no obvious effect on that in 5-min reperfused rats.
CONCLUSION:
The efflux transport of [3H]GABA was markedly enhanced after cerebral ischemia-reperfusion in rats.