Population distributions of allele frequency of apolipoprotein E by age and gender in Han Chinese
Abstract
AIM:
To study apolipoproteins E (ApoE) allele frequency in Han Chinese based on age and gender from Shanghai metropolitan area.
METHODS:
Healthy Han Chinese people (F: 237 and M: 412) were involved in this study. ApoE gene was amplified by PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCT GAG GCC GCG CT-3'. The PCR product was digested directly with 5 units of CfoI and separated by a 20% polyacrylamide nondenaturing gel.
RESULTS:
ApoE*3 was the commonest allele which accounted for 86.4% of the isoforms, and ApoE*2 and ApoE*4 accounted for 6.2% and 7.5%, respectively. The allele and genotype frequencies were in Hardy-Weinberg equilibrium by comparison with that of the corresponding theoretical distribution (P > 0.05).
CONCLUSION:
The frequencies of ApoE*2, ApoE*3, and ApoE*4 were demonstrated in the normal Chinese population.
Keywords:
To study apolipoproteins E (ApoE) allele frequency in Han Chinese based on age and gender from Shanghai metropolitan area.
METHODS:
Healthy Han Chinese people (F: 237 and M: 412) were involved in this study. ApoE gene was amplified by PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCT GAG GCC GCG CT-3'. The PCR product was digested directly with 5 units of CfoI and separated by a 20% polyacrylamide nondenaturing gel.
RESULTS:
ApoE*3 was the commonest allele which accounted for 86.4% of the isoforms, and ApoE*2 and ApoE*4 accounted for 6.2% and 7.5%, respectively. The allele and genotype frequencies were in Hardy-Weinberg equilibrium by comparison with that of the corresponding theoretical distribution (P > 0.05).
CONCLUSION:
The frequencies of ApoE*2, ApoE*3, and ApoE*4 were demonstrated in the normal Chinese population.