Reduction in monocyte chemoattractant protein-1 mRNA expression in peripheral blood mononuclear cells of diamorphine addicts.
Abstract
AIM: To investigate whether there is a significant difference in the monocyte
chemoattractant protein-1 (MCP-1) mRNA expression between diamorphine ( heroin)
addicts and normal volunteers.
METHODS: Expression of MCP-1 mRNA in the peripheral blood mononuclear cells of
diamorphine addicts and normal volunteers was examine d by reverse
transcription-polymerase chain reaction (RT-PCR) with beta -actin as a n internal
standard. Sequencing of RT-PCR products was performed to confirm the specificity
of these products in MCP-1 gene composition.
RESULTS: The relative M CP-1 mRNA expression ratios (MCP-1/beta-actin) in the
peripheral blood mononuclear cells of normal volunteers control group and
diamorphine addicts group were 0.47 +/- 0.12 (n = 15) and 0.21 +/- 0.09 (n =2 1),
respectively, and there was a significant difference (P < 0.05).
CONCLUSION: The significant reduction of MCP-1 mRNA expression in the peripheral
blood mononuclear cells of diamorphine addicts may be one of the mechanisms for
the high incidence o f severe infectious diseases, including AIDS, among
diamorphine addicts.
Keywords:
chemoattractant protein-1 (MCP-1) mRNA expression between diamorphine ( heroin)
addicts and normal volunteers.
METHODS: Expression of MCP-1 mRNA in the peripheral blood mononuclear cells of
diamorphine addicts and normal volunteers was examine d by reverse
transcription-polymerase chain reaction (RT-PCR) with beta -actin as a n internal
standard. Sequencing of RT-PCR products was performed to confirm the specificity
of these products in MCP-1 gene composition.
RESULTS: The relative M CP-1 mRNA expression ratios (MCP-1/beta-actin) in the
peripheral blood mononuclear cells of normal volunteers control group and
diamorphine addicts group were 0.47 +/- 0.12 (n = 15) and 0.21 +/- 0.09 (n =2 1),
respectively, and there was a significant difference (P < 0.05).
CONCLUSION: The significant reduction of MCP-1 mRNA expression in the peripheral
blood mononuclear cells of diamorphine addicts may be one of the mechanisms for
the high incidence o f severe infectious diseases, including AIDS, among
diamorphine addicts.