Inhibitory effects of prostaglandin A1 on apoptosis of rat cardiac microvascular endothelial cells was mediated by NF-kappaB.
Abstract
AIM: To study the effects of prostaglandin A1 (PGA1) on rat cardiac microvascular
endothelial cells. METH-ODS: Isolated rat cardiac microvascular endothelial cells
were cultured in hypoxia and reoxygen conditions, respectively. Endothelial cell
apoptosis was detected by terminal deoxynucleotidyltransferase-mediated dUTP nick
end labeling staining. The activity of NF-kappaB was detected by electrophoretic
mobility shift assay. Bcl-2 and Bax protein expression were examined by Western
blot and bcl-2 mRNA expression was examined by Northern blot.
RESULTS: PGA1 reduced endothelial cell apoptosis markedly, inhibited activity of
NF-kappaB, and increased expression of Bcl-2 protein and bcl-2 mRNA. However,
PGA1 did not alter Bax protein expression resulting in an increase in the ratio
of Bcl-2 to Bax.
CONCLUSION: PGA1 can inhibit rat cardiac microvascular endothelial cell apoptosis
by inhibiting activity of NF-kappaB.
Keywords:
endothelial cells. METH-ODS: Isolated rat cardiac microvascular endothelial cells
were cultured in hypoxia and reoxygen conditions, respectively. Endothelial cell
apoptosis was detected by terminal deoxynucleotidyltransferase-mediated dUTP nick
end labeling staining. The activity of NF-kappaB was detected by electrophoretic
mobility shift assay. Bcl-2 and Bax protein expression were examined by Western
blot and bcl-2 mRNA expression was examined by Northern blot.
RESULTS: PGA1 reduced endothelial cell apoptosis markedly, inhibited activity of
NF-kappaB, and increased expression of Bcl-2 protein and bcl-2 mRNA. However,
PGA1 did not alter Bax protein expression resulting in an increase in the ratio
of Bcl-2 to Bax.
CONCLUSION: PGA1 can inhibit rat cardiac microvascular endothelial cell apoptosis
by inhibiting activity of NF-kappaB.