Expression of intercellular adhesion molecule-1 in U937 foam cells and inhibitory effect of imperatorin.
Abstract
AIM: To investigate the expression level of intercellular adhesion molecule-1
(ICAM-1) in a macrophage-derived foam cell model from human U937 cell line and
the inhibitory effect of imperatorin (IMP) on the ICAM-1.
METHODS: U937 cells were incubated with oxidized low density lipoprotein (ox-LDL)
80 mg/L for 48 h and a macrophage-derived foam cell model was established. The
medium was pretreated with different concentrations of IMP (0, 25, 50, 100
micromol/L). ICAM-1 protein expression in cells was measured with Western
blotting; ICAM-1 mRNA level in cells was measured by Northern blotting.
RESULTS: After incubated with ox-LDL, ICAM-1 expression level increased greatly.
The increase in ICAM-1 protein level and mRNA level was estimated to be about
15-fold and 10-fold. When the cells were pretreated with imperatorin (50, 100
micromol/L), the increase of ICAM-1 in foam cells were remarkably inhibited.
Especially when pretreated with IMP 100 micromol/L, the ICAM-1 protein level
decreased by 79 % and the mRNA level decreased by 74 % each compared to the level
of foam cells.
CONCLUSION: After incubated with ox-LDL in vitro, the U937 foam cells showed an
enhanced ICAM-1 expression compared with normal U937 cells. IMP could inhibit the
expression of ICAM-1 in U937 foam cells.
Keywords:
(ICAM-1) in a macrophage-derived foam cell model from human U937 cell line and
the inhibitory effect of imperatorin (IMP) on the ICAM-1.
METHODS: U937 cells were incubated with oxidized low density lipoprotein (ox-LDL)
80 mg/L for 48 h and a macrophage-derived foam cell model was established. The
medium was pretreated with different concentrations of IMP (0, 25, 50, 100
micromol/L). ICAM-1 protein expression in cells was measured with Western
blotting; ICAM-1 mRNA level in cells was measured by Northern blotting.
RESULTS: After incubated with ox-LDL, ICAM-1 expression level increased greatly.
The increase in ICAM-1 protein level and mRNA level was estimated to be about
15-fold and 10-fold. When the cells were pretreated with imperatorin (50, 100
micromol/L), the increase of ICAM-1 in foam cells were remarkably inhibited.
Especially when pretreated with IMP 100 micromol/L, the ICAM-1 protein level
decreased by 79 % and the mRNA level decreased by 74 % each compared to the level
of foam cells.
CONCLUSION: After incubated with ox-LDL in vitro, the U937 foam cells showed an
enhanced ICAM-1 expression compared with normal U937 cells. IMP could inhibit the
expression of ICAM-1 in U937 foam cells.