Inhibitory effect of melittin on Na+,K+-ATPase from guinea pig myocardial mitochondria
Abstract
Aim: To investigate the effect of melittin (Mel) of Na+,K+-ATPase activity and it's kinetic mode of action on guinea pig myocardial mitochondria.
Methods: Effect of Mel on heart mitochondrial Na+,K+-ATPase activity was determined with colorimetry method.
Results: Mel inhibited Na+,K+-ATPase in a concentration and time dependent manner, IC50 was 2.60 micromol/L. Kinetic studies of interaction between Mel and K+, Na+, ATP revealed that inhibitory effect of Mel was competitive with K+, but not with Na+ and ATP.
Conclusion: Mel polypeptide potently inhibits Na+,K+-ATPase, possibly by binding to the K+ site.
Keywords:
Methods: Effect of Mel on heart mitochondrial Na+,K+-ATPase activity was determined with colorimetry method.
Results: Mel inhibited Na+,K+-ATPase in a concentration and time dependent manner, IC50 was 2.60 micromol/L. Kinetic studies of interaction between Mel and K+, Na+, ATP revealed that inhibitory effect of Mel was competitive with K+, but not with Na+ and ATP.
Conclusion: Mel polypeptide potently inhibits Na+,K+-ATPase, possibly by binding to the K+ site.