Effects of hyperin on free intracellular calcium in dissociated neonatal rat brain cells
Abstract
AIM:
To study the effects of hyperin (Hyp) on free intracellular calcium concentration ([Ca2+]i) of brain cells.
METHODS:
The neonatal rat brain cells were dissociated. [Ca2+]i in presence and absence of extracellular high K+, L-glutamic acid (Glu), 5-hydroxytryptamine (5-HT), and norepinephrine (NE) were assayed with Fura 2-AM.
RESULTS:
The resting [Ca2+]i in Hanks' solution (CaCl2 1.3 mmol.L-1) was (208 +/- 12) nmol.L-1 (n = 17). Hyp had no significant effects on the resting [Ca2+]i. Hyp 1.0, 4.0, and 16.0 mumol.L-1 markedly inhibited the increase of [Ca2+]i evoked by K+ 50 mmol.L-1 in a concentration-dependent manner. Hyp 16.0 mumol.L-1 inhibited the increases of [Ca2+]i induced by NE 1, 2, 4, and 8 mumol.L-1. Hyp (16.0 mumol.L-1) also markedly attenuated 5-HT and Glu-induced increase of [Ca2+]i.
CONCLUSION:
Hyp possessed inhibitory effects on influx of Ca2+ in the neonatal rat brain cells.
Keywords:
To study the effects of hyperin (Hyp) on free intracellular calcium concentration ([Ca2+]i) of brain cells.
METHODS:
The neonatal rat brain cells were dissociated. [Ca2+]i in presence and absence of extracellular high K+, L-glutamic acid (Glu), 5-hydroxytryptamine (5-HT), and norepinephrine (NE) were assayed with Fura 2-AM.
RESULTS:
The resting [Ca2+]i in Hanks' solution (CaCl2 1.3 mmol.L-1) was (208 +/- 12) nmol.L-1 (n = 17). Hyp had no significant effects on the resting [Ca2+]i. Hyp 1.0, 4.0, and 16.0 mumol.L-1 markedly inhibited the increase of [Ca2+]i evoked by K+ 50 mmol.L-1 in a concentration-dependent manner. Hyp 16.0 mumol.L-1 inhibited the increases of [Ca2+]i induced by NE 1, 2, 4, and 8 mumol.L-1. Hyp (16.0 mumol.L-1) also markedly attenuated 5-HT and Glu-induced increase of [Ca2+]i.
CONCLUSION:
Hyp possessed inhibitory effects on influx of Ca2+ in the neonatal rat brain cells.