Effect of diet supplementation with l-carnitine on hepatic catabolism of l-alanine in rats.
Abstract
AIM: To investigate the effects of chronic supplementation with l-carnitine (LCT)
on hepatic catabolism of l-alanine.
METHODS: Two groups of male adult Wistar rats were used: 1) supplemented with LCT
(1.2 mmol . kg-1 . d-1) dissolved in the drinking water (LCT group) and 2)
control group (COG) without LCT supplementation. After one week of LCT
supplementation livers from 24 h-fasted rats were perfused in situ and the
production of glucose, urea, pyruvate, and l-lactate from l-alanine (5 mmol/L)
were measured.
RESULTS: LCT decreased the production of glucose and urea from l-alanine. In
agreement, pyruvate and l-lactate production from l-alanine were decreased.
However, the supplementation with LCT did not show any significant effect on
hepatic glucose production from pyruvate (5 mmol/L) and l-lactate (2 mmol/L).
CONCLUSION: LCT supplementation decreased the conversion of l-alanine
Keywords:
on hepatic catabolism of l-alanine.
METHODS: Two groups of male adult Wistar rats were used: 1) supplemented with LCT
(1.2 mmol . kg-1 . d-1) dissolved in the drinking water (LCT group) and 2)
control group (COG) without LCT supplementation. After one week of LCT
supplementation livers from 24 h-fasted rats were perfused in situ and the
production of glucose, urea, pyruvate, and l-lactate from l-alanine (5 mmol/L)
were measured.
RESULTS: LCT decreased the production of glucose and urea from l-alanine. In
agreement, pyruvate and l-lactate production from l-alanine were decreased.
However, the supplementation with LCT did not show any significant effect on
hepatic glucose production from pyruvate (5 mmol/L) and l-lactate (2 mmol/L).
CONCLUSION: LCT supplementation decreased the conversion of l-alanine