Huperzine B protects rat pheochromocytoma cells against oxygen-glucose deprivation-induced injury.
Abstract
AIM: To test the ability of huperzine B (HupB) to alleviate injury from
oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells.
METHODS: After OGD for 3 h and reoxygenation for 24 h, neuronal morphology was
observed by phase-contrast microscopy; cell survival was quantified by the
reduction of MTT; malondialdehyde (MDA) was determined by the thiobarbituric
acid; superoxide dismutase (SOD) was assayed by a modification of the
xanthine/xanthine oxidase; and lactate (LA) was measured according to Marbach and
Weil.
RESULTS: OGD for 3 h and reoxygenation for 24 h triggered death in nearly 70 % of
cells, along with major changes in morphology and biochemistry including elevated
level of MDA, SOD activity, and LA content. Cells pretreated with HupB 1-100
micromol/L for 2 h showed significantly improved survival and reduced biochemical
and morphologic signs of toxicity.
CONCLUSION: HupB protected PC12 cells against OGD-induced injury, most likely by
alleviating disturbances of oxidative and energy metabolism.
Keywords:
oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells.
METHODS: After OGD for 3 h and reoxygenation for 24 h, neuronal morphology was
observed by phase-contrast microscopy; cell survival was quantified by the
reduction of MTT; malondialdehyde (MDA) was determined by the thiobarbituric
acid; superoxide dismutase (SOD) was assayed by a modification of the
xanthine/xanthine oxidase; and lactate (LA) was measured according to Marbach and
Weil.
RESULTS: OGD for 3 h and reoxygenation for 24 h triggered death in nearly 70 % of
cells, along with major changes in morphology and biochemistry including elevated
level of MDA, SOD activity, and LA content. Cells pretreated with HupB 1-100
micromol/L for 2 h showed significantly improved survival and reduced biochemical
and morphologic signs of toxicity.
CONCLUSION: HupB protected PC12 cells against OGD-induced injury, most likely by
alleviating disturbances of oxidative and energy metabolism.