Protective effects of polypeptide from Chlamys farreri on Hela cells damaged by ultraviolet A.
Abstract
AIM: To study the protective effect of polypeptide isolated from Chlamys farreri
(PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro.
METHODS: Cell proliferation was determined by MTT method; intra-cellular free
calcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry
(FCM).
RESULTS: PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase
(GSH-px), superoxide dismutase (SOD), and catalase (CAT), and stimulated cell
proliferation. The concentration of [Ca2+]i was increased while the amounts of
MDA and the rates of apoptosis and death of the cells were decreased. The
differences between the PCF groups and control group were significant (P<0.05,
P<0.01).
CONCLUSION: PCF protected Hela cells against damage by UVA via its anti-oxidative
mechanisms.
Keywords:
(PCF) on Hela cells damaged by ultraviolet A (UVA) in vitro.
METHODS: Cell proliferation was determined by MTT method; intra-cellular free
calcium [Ca2+]i and rates of apoptosis and death were measured by flow cytometry
(FCM).
RESULTS: PCF (0.5 %-2 %) enhanced the activities of glutathione peroxidase
(GSH-px), superoxide dismutase (SOD), and catalase (CAT), and stimulated cell
proliferation. The concentration of [Ca2+]i was increased while the amounts of
MDA and the rates of apoptosis and death of the cells were decreased. The
differences between the PCF groups and control group were significant (P<0.05,
P<0.01).
CONCLUSION: PCF protected Hela cells against damage by UVA via its anti-oxidative
mechanisms.