Effects of resveratrol and ethanol on production of pro-inflammatory factors from endotoxin activated murine macrophages.

AIM: To study the interaction of resveratrol and ethanol on the production of
pro-inflammatory factors from activated murine peritoneal macrophages (MPM).
METHODS: NO production was measured with Griess assay; IL-1 production was
measured through thymocyte co-stimulating assay; IL-6 and TNF-alpha were detected
by ELISA method.
RESULTS: Resveratrol (6.25, 12.5, 25 micromol/L) and ethanol (0.2 %, 0.8 %)
synergistically inhibited the 24 h production of NO from lipopolysaccharide (LPS,
1 mg/L) and IFN-gamma (5 kU/L) stimulated MPM; resveratrol at higher dose (25
micromol/L) also inhibited IL-6 production. Ethanol additively strengthened this
effect. Ethanol had no significant influence on 24 h MPM IL-1 production, but it
promoted the ability of resveratrol on enhancing the IL-1 release from activated
MPM. Low doses of ethanol inhibited 24 h production of TNF-alpha, however, both
dose of ethanol enhanced the promoting effect of resveratrol on TNF-alpha
production.
CONCLUSION: Resveratrol and ethanol can interact to influence the production of
macrophage function molecules, which is noteworthy in evaluating the health-care
effect of wine consumption.