Effect of astragaloside IV on T, B lymphocyte proliferation and peritoneal macrophage function in mice.

AIM: To investigate the effect of astragaloside IV (ASI) on T, B lymphocyte
proliferation, antibody production, and cytokines produced by murine peritoneal
macrophages.
METHODS: MTT assay was used to determine T, B lymphocyte proliferation and
quantitative hemolysin spectrophotometry (QHS) assay was applied to test antibody
production; IL-1 production was measured by thymocyte proliferation assay;
TNF-alpha production was determined by the cytotoxicity assay against L929 cells.
RESULTS: 1) In vivo, ASI 50-200 mg/kg ig for 7 d increased T lymphocyte
proliferation and antibody production, and ASI 50-100 mg/kg ig for 7 d increased
B lymphocyte proliferation but ASI 200 mg/kg had no effect on B lymphocyte
proliferation; 2) In vitro, ASI increased T, B lymphocyte proliferation only at
100 nmol/L; 3) ASI increased IL-1 activity at 1 nmol/L in vitro, but decreased it
at 100 and 1000 nmol/L; 4) ASI inhibited TNF-alpha activity with or without
LPS-stimulation in vitro.
CONCLUSION: ASI increased T, B lymphocyte proliferation and antibody production
in vivo and in vitro; but inhibited productions of IL-1 and TNF-alpha from
peritoneal macrophages in vitro.