Nonassociation of aryl hydrocarbon receptor genotypes with susceptibility to bladder cancer in Shanghai population.
Abstract
AIM: To assess two polymorphic forms of aryl hydrocarbon receptor (AHR) gene,
G1721A (R554K), and G1768A (V570I) in Chinese population and to explore the
possible association of human AHR gene polymorphism with elevated incidence of
bladder cancer among Chinese Han subjects in east of China.
METHODS: An allele specific PCR-based procedure for AHR gene polymorphism
genotyping was developed by this work. Genotyping on three groups of subjects in
Shanghai area had been performed: a bladder cancer group with the occupational
exposure to benzidine, a non-occupational bladder cancer patient group whose
members lack an obvious aromatic amine exposure record, and a normal population
in the same city as controls.
RESULTS: A significant difference (P < 0.01) in frequency distribution at locus
G1721A between normal population in Shanghai and a Caucasian population reported
by other authors was observed. No mutant allele(A1768) at locus G1768A had ever
been detected in our study. The observed frequencies were similar between both
genders in the normal population (P = 0.54), and there were no significant
difference confirmed between the case group and the control group.
CONCLUSION: The locus G1768 of human AHR gene seems to be monomorphic among
Chinese in this area. The significant distribution difference at locus G1721A of
human AHR gene between Chinese Han and Caucasian was confirmed. This study did
not support the association of AHR G1721A polymorphism with higher risk to
bladder cancer among the residents in this area, either in a group of
occupationally benzidine-exposed individuals or among the persons who never have
an obvious aromatic amine exposure record.
Keywords:
G1721A (R554K), and G1768A (V570I) in Chinese population and to explore the
possible association of human AHR gene polymorphism with elevated incidence of
bladder cancer among Chinese Han subjects in east of China.
METHODS: An allele specific PCR-based procedure for AHR gene polymorphism
genotyping was developed by this work. Genotyping on three groups of subjects in
Shanghai area had been performed: a bladder cancer group with the occupational
exposure to benzidine, a non-occupational bladder cancer patient group whose
members lack an obvious aromatic amine exposure record, and a normal population
in the same city as controls.
RESULTS: A significant difference (P < 0.01) in frequency distribution at locus
G1721A between normal population in Shanghai and a Caucasian population reported
by other authors was observed. No mutant allele(A1768) at locus G1768A had ever
been detected in our study. The observed frequencies were similar between both
genders in the normal population (P = 0.54), and there were no significant
difference confirmed between the case group and the control group.
CONCLUSION: The locus G1768 of human AHR gene seems to be monomorphic among
Chinese in this area. The significant distribution difference at locus G1721A of
human AHR gene between Chinese Han and Caucasian was confirmed. This study did
not support the association of AHR G1721A polymorphism with higher risk to
bladder cancer among the residents in this area, either in a group of
occupationally benzidine-exposed individuals or among the persons who never have
an obvious aromatic amine exposure record.