Effect of mifepristone on uterine receptivity in guinea pigs.
Abstract
AIM: To determine the effect of a low-dose mifepristone regimen on endometrial
receptivity in guinea pigs.
METHODS: Histologic evaluation for endometrial staging and immunoreactivity of
cytokines [leukemia inhibitory factor (LIF) and interleukin-6 (IL-6)] were
preformed on fixed endometrial tissue, and relative abundance of endometrial
estrogen (E), and progesterone (P) receptor mRNA were evaluated with
semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) in
which cyclophilin mRNA, a housekeeping gene product, was co-amplified as the
reference standard.
RESULTS: Mifepristone at 0.03 mg.kg-1 . d-1 induced a delay in endometrial
maturation. The staining intensity of LIF and IL-6 decreased in treated guinea
pigs. E and P receptor mRNA increased significantly and uterine stage delayed in
treated guinea pigs.
CONCLUSION: Mifepristone at 0.03 mg.kg-1 . d-1 delayed development of
endometrium. This study provides further evidence that endometrial maturation can
be altered without affecting ovarian ovulatory function.
Keywords:
receptivity in guinea pigs.
METHODS: Histologic evaluation for endometrial staging and immunoreactivity of
cytokines [leukemia inhibitory factor (LIF) and interleukin-6 (IL-6)] were
preformed on fixed endometrial tissue, and relative abundance of endometrial
estrogen (E), and progesterone (P) receptor mRNA were evaluated with
semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) in
which cyclophilin mRNA, a housekeeping gene product, was co-amplified as the
reference standard.
RESULTS: Mifepristone at 0.03 mg.kg-1 . d-1 induced a delay in endometrial
maturation. The staining intensity of LIF and IL-6 decreased in treated guinea
pigs. E and P receptor mRNA increased significantly and uterine stage delayed in
treated guinea pigs.
CONCLUSION: Mifepristone at 0.03 mg.kg-1 . d-1 delayed development of
endometrium. This study provides further evidence that endometrial maturation can
be altered without affecting ovarian ovulatory function.