Inhibitory effects of praeruptorin C on cattle aortic smooth muscle cell proliferation.
Abstract
AIM: To study the effects of praeruptorin C (pra-C) on proliferation of cattle
aortic smooth muscle cells (SMC).
METHODS: The DNA synthesis of SMC was measured using the incorporation of
[3H]thymidine([3H]TdR). Cell cycle phase was evaluated by flow cytometry and
cytotoxicity was evaluated by measuring lactic dehydrogenase (LDH) activity.
RESULTS: Whether or not treated with angiotensin II (Ang-II), SMC proliferation
was suppressed by pra-C in a concentration-dependent manner at range from 0.001
micromol/L to 10 micromol/L. The inhibitory effects appeared to be related to
G1-S block in cell cycle traverse while the LDH activities did not change
dramatically.
CONCLUSION: Pra-C can completely inhibit SMC proliferation induced by Ang II and
partly inhibit the growth of SMC- induced by bovine serum, which is important in
prevention and treatment of vascular hyperplastic disease.
Keywords:
aortic smooth muscle cells (SMC).
METHODS: The DNA synthesis of SMC was measured using the incorporation of
[3H]thymidine([3H]TdR). Cell cycle phase was evaluated by flow cytometry and
cytotoxicity was evaluated by measuring lactic dehydrogenase (LDH) activity.
RESULTS: Whether or not treated with angiotensin II (Ang-II), SMC proliferation
was suppressed by pra-C in a concentration-dependent manner at range from 0.001
micromol/L to 10 micromol/L. The inhibitory effects appeared to be related to
G1-S block in cell cycle traverse while the LDH activities did not change
dramatically.
CONCLUSION: Pra-C can completely inhibit SMC proliferation induced by Ang II and
partly inhibit the growth of SMC- induced by bovine serum, which is important in
prevention and treatment of vascular hyperplastic disease.