Inhibition of 11beta-hydroxysteroid dehydrogenase in guinea pig kidney by three bioflavonoids and their interactions with gossypol.
Abstract
AIM: To study the effects of some bioflavonoids on the gossypol-induced
hypokalemia.
METHODS: The 11beta-hydroxysteroid dehydrogenase (11beta-OHSD) protein was
prepared from guinea pig kidney. The activity of 11beta-OHSD with NAD as the
coenzyme was measured by HPLC. The drug interaction was analysed by
isobolographic method.
RESULTS: The 11beta-OHSD can be inhibited by some bioflavonoids. The IC50 (95 %
confidence limits) values were: quercetin 164 (79 - 341) micromol/L, morin 913
(385 - 2173) micromol/L, and naringenin 2193 (1114 - 4315) micromol/L. When the
11beta-OHSD was treated with quercetin, tangeretin, morin, naringenin plus
gossypol, the combination index (CI) values were 0.92, 0.85, 0.98, and 1.01
respectively.
CONCLUSION: The interaction of some bioflavonoids with gossypol might be one of
the factors for gossypol-induced hypokalemia.
Keywords:
hypokalemia.
METHODS: The 11beta-hydroxysteroid dehydrogenase (11beta-OHSD) protein was
prepared from guinea pig kidney. The activity of 11beta-OHSD with NAD as the
coenzyme was measured by HPLC. The drug interaction was analysed by
isobolographic method.
RESULTS: The 11beta-OHSD can be inhibited by some bioflavonoids. The IC50 (95 %
confidence limits) values were: quercetin 164 (79 - 341) micromol/L, morin 913
(385 - 2173) micromol/L, and naringenin 2193 (1114 - 4315) micromol/L. When the
11beta-OHSD was treated with quercetin, tangeretin, morin, naringenin plus
gossypol, the combination index (CI) values were 0.92, 0.85, 0.98, and 1.01
respectively.
CONCLUSION: The interaction of some bioflavonoids with gossypol might be one of
the factors for gossypol-induced hypokalemia.