Original Article

Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells

Hong-Li Peng, Dao-Feng Chen, Hong-Xiang Lan, Xue-Mei Zhang, Zheng Gu, Ming-Hua Jiang

Abstract

AIM: To study the influences of gomisin J on lipid peroxidation and calcium paradox.
METHODS: Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells.
RESULTS: Gomisin J inhibited Fe2+/ascorbic acid and ADP/NADPH-induced LPO with IC50 (95% confidence limits) 5.5 (4.5-6.7) and 4.7 (2.8-7.8) mumol.L-1, respectively, when cultured myocardial cells preincubated with Ca(2+)-free medium for 2 min were incubated with normal medium containing Ca2+, a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 mumol.L-1 protected myocardial cells through decreasing MDA formation.
CONCLUSION: Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injured by calcium paradox
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