Effects of nicorandil on spontaneous beating and action potentials of cultured myocardial cells of neonatal rats
Abstract
AIM:
To study the anti-arrhythmic mechanism of nicorandil (Nic).
METHODS:
The myocardial cells of neonatal rats were cultured and effects of Nic on spontaneous beating and action potential of cultured myocardial cells were observed.
RESULTS:
Nic 32 mumol L-1 and 160 mumol L-1 decreased the spontaneous beating rates of cultured myocardial cells of neonatal rats. Nic 64 mumol L-1 prevented the beating rats induced by CaCl2 1 mmol L-1 and isoproterenol 2 mumol L-1. Nic 32 and 160 mumol L-1 reduced the amplitude, maximal rate of rise, and shortened APD50 and APD90 repolarization. Nic prolonged the spontaneous sinus cycle length.
CONCLUSION:
The antiarrhythmic mechanism of Nic is related to its direct effects on the myocardial cells.
Keywords:
To study the anti-arrhythmic mechanism of nicorandil (Nic).
METHODS:
The myocardial cells of neonatal rats were cultured and effects of Nic on spontaneous beating and action potential of cultured myocardial cells were observed.
RESULTS:
Nic 32 mumol L-1 and 160 mumol L-1 decreased the spontaneous beating rates of cultured myocardial cells of neonatal rats. Nic 64 mumol L-1 prevented the beating rats induced by CaCl2 1 mmol L-1 and isoproterenol 2 mumol L-1. Nic 32 and 160 mumol L-1 reduced the amplitude, maximal rate of rise, and shortened APD50 and APD90 repolarization. Nic prolonged the spontaneous sinus cycle length.
CONCLUSION:
The antiarrhythmic mechanism of Nic is related to its direct effects on the myocardial cells.