Effect of morphine on interleukin-1 and tumor necrosis factor alpha production from mouse peritoneal macrophages in vitro
Abstract
AIM:
To study the influence of morphine on lymphokine production from mouse peritoneal macrophages (PM phi).
METHODS:
The productions of interleukin-1 (IL-1) and tumor necrosis factor (TNF-alpha) from PM phi under various concentrations of morphine with or without naloxone were measured by testing thymocyte proliferation and L929 cell lysis in vitro.
RESULTS:
Both morphine (1 x 10(-8) - 1 x 10(-3) mol L-1) and naloxone (50 mumol L-1) inhibited IL-1 production from mouse PM phi primed by sodium thioglycolate. Naloxone did not block the inhibitory effect of morphine. TNF-alpha production from PM phi was inhibited by only high concentration (1 mmol L-1) of morphine which was not blocked by naloxone.
CONCLUSION:
Morphine had a direct inhibitory effect on PM phi, which was not mediated by opioid receptor of PM phi.
Keywords:
To study the influence of morphine on lymphokine production from mouse peritoneal macrophages (PM phi).
METHODS:
The productions of interleukin-1 (IL-1) and tumor necrosis factor (TNF-alpha) from PM phi under various concentrations of morphine with or without naloxone were measured by testing thymocyte proliferation and L929 cell lysis in vitro.
RESULTS:
Both morphine (1 x 10(-8) - 1 x 10(-3) mol L-1) and naloxone (50 mumol L-1) inhibited IL-1 production from mouse PM phi primed by sodium thioglycolate. Naloxone did not block the inhibitory effect of morphine. TNF-alpha production from PM phi was inhibited by only high concentration (1 mmol L-1) of morphine which was not blocked by naloxone.
CONCLUSION:
Morphine had a direct inhibitory effect on PM phi, which was not mediated by opioid receptor of PM phi.