Effects of tetrandrine and chlorpromazine on synthesis of collagen and hyaluronic acid in cultured human lung fibroblasts
Abstract
AIM:
To study the effects of tetrandrine (Tet) and chlorpromazine (Chl) on synthesis of collagen and hyaluronic acid (HA) in cultured human lung fibroblasts (HLF).
METHODS:
The synthesis of collagen and HA was assessed by measuring the incorporation of [3H]proline and radioimmunoassay.
RESULTS:
Both Tet (5-80 mumol L-1) and Chl (10-40 mumol L-1) diminished the collagen synthesis in a concentration-dependent manner. The suppression was aggravated at 36-48 h. The HA content in the supernatant of culture also decreased gradually with the increasing dosage of Tet or Chl after 24-h exposure. There was no obvious toxic effect of Tet on HLF cells at 5-20 mumol L-1.
CONCLUSION:
Tet 5-20 mumol L-1 decreased the production of collagen and HA without obvious toxity on HLF, suggesting that Tet could be a hopeful anti-fibrosis drug.
Keywords:
To study the effects of tetrandrine (Tet) and chlorpromazine (Chl) on synthesis of collagen and hyaluronic acid (HA) in cultured human lung fibroblasts (HLF).
METHODS:
The synthesis of collagen and HA was assessed by measuring the incorporation of [3H]proline and radioimmunoassay.
RESULTS:
Both Tet (5-80 mumol L-1) and Chl (10-40 mumol L-1) diminished the collagen synthesis in a concentration-dependent manner. The suppression was aggravated at 36-48 h. The HA content in the supernatant of culture also decreased gradually with the increasing dosage of Tet or Chl after 24-h exposure. There was no obvious toxic effect of Tet on HLF cells at 5-20 mumol L-1.
CONCLUSION:
Tet 5-20 mumol L-1 decreased the production of collagen and HA without obvious toxity on HLF, suggesting that Tet could be a hopeful anti-fibrosis drug.