Effect of beta-carotene on 60Co-gamma-induced mutation at T-lymphocyte hypoxanthine-guanine phosphoribosyl transferase locus in rats
Abstract
AIM: To study the effect of beta-carotene (beta-Car) on radiation-induced
mutation.
METHODS: Mutant frequency at T-lymphocyte hypoxanthine-guanine phosphoribosyl
transferase (HGPRT) locus in rats was measured using a T-lymphocyte cloning
technique and malondialdehyde (MDA) was measured using a thiobarbituric acid
(TBA).
RESULTS: Mutant frequency at the HGPRT locus was elevated by 60Co-gamma 3.75 Gy,
and was reduced by ig beta-car 20 and 10 mg.kg-1 (P < 0.05), but not by beta-Car
5 mg.kg-1. It showed a dose-dependent relationship. MDA was signifcantly reduced
in plasma of rats given beta-Car after 60 Co-gamma radiation. There was positive
coherence between MDA and mutant frequency at the HGPRT locus (r = 0.9978, P <
0.05).
CONCLUSIONS: Radiation-induced mutation is inhibited by beta-Car, associated with
antioxidant action of beta-Car.
Keywords:
mutation.
METHODS: Mutant frequency at T-lymphocyte hypoxanthine-guanine phosphoribosyl
transferase (HGPRT) locus in rats was measured using a T-lymphocyte cloning
technique and malondialdehyde (MDA) was measured using a thiobarbituric acid
(TBA).
RESULTS: Mutant frequency at the HGPRT locus was elevated by 60Co-gamma 3.75 Gy,
and was reduced by ig beta-car 20 and 10 mg.kg-1 (P < 0.05), but not by beta-Car
5 mg.kg-1. It showed a dose-dependent relationship. MDA was signifcantly reduced
in plasma of rats given beta-Car after 60 Co-gamma radiation. There was positive
coherence between MDA and mutant frequency at the HGPRT locus (r = 0.9978, P <
0.05).
CONCLUSIONS: Radiation-induced mutation is inhibited by beta-Car, associated with
antioxidant action of beta-Car.