Serum alpha 1-acid glycoprotein, sialic acid, and protein binding of disopyramide in normal subjects and cardiac patients
Abstract
AIM: To study influence of congestive heart failure (CHF) and acute myocardial
infarction (AMI) on alpha 1-acid glycoprotein (AGP) and sialic acid (SA)
concentration, and binding of AGP to disopyramide (Dis).
METHODS: Sera from 85 healthy subjects, 6 patients with CHF, and 6 patients with
AMI were determined by immunochemistry for AGP, by HPLC method for sialic acid
(SA), and by ultrafiltration and HPLC for the free fraction of Dis.
RESULTS: Serum AGP concentrations (g.L-1) were 0.74 +/- 0.16 (healthy), 1.18 +/-
0.40 (d 1, CHF) and 0.90 +/- 0.24 (d 14, CHF), 1.53 +/- 0.26 (d 5, AMI) and 1.08
(d 14, AMI). The free Dis were 1.76 +/- 0.62 (d 1) and 2.14 +/- 0.48 (d 14), in
CHF patients, 1.66 +/- 0.52 (d 5) and 1.77 (d 14) in AMI patients. The changes of
serum SA and AGP concentrations showed the same tendency.
CONCLUSION: The free Dis in serum was affected by the change of AGP binding in
CHF and AMI patients.
Keywords:
infarction (AMI) on alpha 1-acid glycoprotein (AGP) and sialic acid (SA)
concentration, and binding of AGP to disopyramide (Dis).
METHODS: Sera from 85 healthy subjects, 6 patients with CHF, and 6 patients with
AMI were determined by immunochemistry for AGP, by HPLC method for sialic acid
(SA), and by ultrafiltration and HPLC for the free fraction of Dis.
RESULTS: Serum AGP concentrations (g.L-1) were 0.74 +/- 0.16 (healthy), 1.18 +/-
0.40 (d 1, CHF) and 0.90 +/- 0.24 (d 14, CHF), 1.53 +/- 0.26 (d 5, AMI) and 1.08
(d 14, AMI). The free Dis were 1.76 +/- 0.62 (d 1) and 2.14 +/- 0.48 (d 14), in
CHF patients, 1.66 +/- 0.52 (d 5) and 1.77 (d 14) in AMI patients. The changes of
serum SA and AGP concentrations showed the same tendency.
CONCLUSION: The free Dis in serum was affected by the change of AGP binding in
CHF and AMI patients.