Melatonin decreases production of hydroxyl radical during cerebral ischemia-reperfusion
Abstract
AIM: To study the effect of melatonin on hydroxyl radical (.OH) contents during
cerebral ischemia-reperfusion in rats.
METHODS: Ischemia was induced by occluding left lateral middle cerebral artery
for 30 min following reperfusion. The salicylate trapping method coupled with
ipsilateral striatal microdialysis for measurement of hydroxyl radicals generated
during ischemia and reperfusion.
RESULTS: The contents of dihydroxybenzoic acid (DHBA) were increased at 15 min
after ischemia and remained high for 30 min after reperfusion. Melatonin (4
mg.kg-1, sc, 30 min before ischemia) decreased the production of DHBA during
ischemia for 16-30 min and reperfusion for 1-30 min.
CONCLUSION: Melatonin inhibits the production of hydroxyl radicals in rat brain
during ischemia and reperfusion.
Keywords:
cerebral ischemia-reperfusion in rats.
METHODS: Ischemia was induced by occluding left lateral middle cerebral artery
for 30 min following reperfusion. The salicylate trapping method coupled with
ipsilateral striatal microdialysis for measurement of hydroxyl radicals generated
during ischemia and reperfusion.
RESULTS: The contents of dihydroxybenzoic acid (DHBA) were increased at 15 min
after ischemia and remained high for 30 min after reperfusion. Melatonin (4
mg.kg-1, sc, 30 min before ischemia) decreased the production of DHBA during
ischemia for 16-30 min and reperfusion for 1-30 min.
CONCLUSION: Melatonin inhibits the production of hydroxyl radicals in rat brain
during ischemia and reperfusion.