Effect of tetrandrine on proto-oncogene c-fos expression in rat cerebrum
Abstract
AIM: To detect the effect of tetrandrine (Tet) on c-fos gene expression in
cerebrum induced by lindane, a neurotoxicant which activates Ca2+ channels.
METHODS: Northern and dot blotting, dual wavelength thin layer chromatography
scanner, were used in this study.
RESULTS: Lindane 30 mg.kg-1 given by intragastric gavage (i.g.) increased the
expression of c-fos gene to 146 mm2 in rat cerebrum 1 h after treatment. Tet 1,
2, and 4 mg.kg-1 given by i.g. 30 min prior to lindane reduced c-fos gene
expression in a concentration-dependent manner. Expressed genes reached only 86,
40, and 39 mm2, respectively.
CONCLUSION: Tet inhibited c-fos gene expression in rat cerebrum induced by Ca2+
agonist-lindane.
Keywords:
cerebrum induced by lindane, a neurotoxicant which activates Ca2+ channels.
METHODS: Northern and dot blotting, dual wavelength thin layer chromatography
scanner, were used in this study.
RESULTS: Lindane 30 mg.kg-1 given by intragastric gavage (i.g.) increased the
expression of c-fos gene to 146 mm2 in rat cerebrum 1 h after treatment. Tet 1,
2, and 4 mg.kg-1 given by i.g. 30 min prior to lindane reduced c-fos gene
expression in a concentration-dependent manner. Expressed genes reached only 86,
40, and 39 mm2, respectively.
CONCLUSION: Tet inhibited c-fos gene expression in rat cerebrum induced by Ca2+
agonist-lindane.