Kinetic properties of nicotinic receptors in cultured rat sympathetic neurons from superior cervical ganglia
Abstract
AIM: To analyze the kinetic properties of the effect of nicotine on nicotinic
acetylcholine receptors (nAChR) in the cultured sympathetic neurons from neonatal
rat superior cervical ganglia (SCG).
METHODS: The whole-cell recording method of patch-clamp technique was used to
record the currents induced by different concentrations of nicotine. The
concentration-response of nicotine was fitted with Clark equation.
RESULTS: Hill coefficient (1.097) was determined by fitting the nicotine
responses of neuronal nAChR with Clark equation. The theoretical values of
nicotine effect, calculated with Clark equation with H = 1, were basically
identical with the practically recorded currents.
CONCLUSIONS: Interaction of nicotine and nAChR in rat SCG fits a single binding
site model.
Keywords:
acetylcholine receptors (nAChR) in the cultured sympathetic neurons from neonatal
rat superior cervical ganglia (SCG).
METHODS: The whole-cell recording method of patch-clamp technique was used to
record the currents induced by different concentrations of nicotine. The
concentration-response of nicotine was fitted with Clark equation.
RESULTS: Hill coefficient (1.097) was determined by fitting the nicotine
responses of neuronal nAChR with Clark equation. The theoretical values of
nicotine effect, calculated with Clark equation with H = 1, were basically
identical with the practically recorded currents.
CONCLUSIONS: Interaction of nicotine and nAChR in rat SCG fits a single binding
site model.