Attenuation of myocardial injury due to oxygen free radicals (OFR) by pretreatment with OFR or calcitonin gene-related peptide

AIM: To study the cardioprotective effects of oxygen free radicals (OFR) and
calcitonin gene-related peptide (OGRP) pretreatment on myocardial damages due to
OFR in isolated perfused rat heart.
METHODS: The hearts were perfused in a Langendorff mode. OFR were generated by
electrolysis of Krebs-Henseleit (K-H) solution.
RESULTS: OFR pretreatment reduced the impairment of cardiac contractile function,
the decrease of coronary flow and the increase of creatinine kinase (CK) release
due to OFR, and the effect exhibited period dependence and cycle-dependence.
1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), an inhibitor of protein
kinase C, abolished the protection of OFR pretreatment (CK release = 110 +/- 7,
215 +/- 23, 169 +/- 14, 240 +/- 30, and 113 +/- 19 kU.L-1 for control, OFR, OFR
pretreatment, OFR pretreatment plus H-7, and H-7, respectively). CGRP
pretreatment also protected the myocardium damages elicited by OFR in isolated
perfused rat heart.
CONCLUSIONS: OFR or CGRP pretreatment protected myocardium against injury
elicited by OFR, and the effect of OFR pretreatment was related to the activation
of PKC.