Effects of isolation housing and timing of drug administration on amikacin kinetics in mice

AIM: To study the influences of social condition and drug administration time on
amikacin metabolism in mice.
METHODS: Forty Male ICR mice were randomly assigned into 4 groups according to 1)
housing condition: individual housing (I, one mouse in a cage) or aggregated
housing (A, 10 mice in a cage) and 2) drug administration time: at midday (D) or
at midnight (N), i.e. I-D, I-N, A-D, and A-N groups. Amikacin was injected s.c.
15 mg.kg-1 after 4 wk of raising at D or N. Blood samples were taken at 5, 10,
15, 20, 30, and 60 min after medication in each mouse. Plasma amikacin was
measured by enzyme immunoassay. The concentration-time data were fitted with
one-compartment open model in each mouse and data were analyzed with group t
test.
RESULTS: The clearance (Cl) of amikacin was larger and the half-life (T1/2) was
shorter in A-N group than in A-D or I-N groups respectively. AUC(0-1) in A-N
group was less than in I-N group. No differences of kinetic parameters between 2
isolated housing (I-D and I-N) groups were found.
CONCLUSION: Aggregated housing and midnight drug administration increased the
disposition of amikacin.