Quercetin induced apoptosis in human leukemia HL-60 cells.
Abstract
AIM: To examine whether quercetin (Que) might induce apoptosis in human leukemia
HL-60 cells.
METHODS: DNA fragmentation was visualized by agarose gel electrophoresis.
Inhibition of proliferation was measured with a colorimetric MTT-assay. The DNA
degradation was determined using flow cytometry, and the microscopic changes were
observed by an electron microscope.
RESULTS: Que 15-120 mumol.L-1 elicited typical apoptosis morphological changes
including condensed chromatin, nuclear fragmentation, and reduction in volume.
DNA fragmentation and DNA degradation in a concentration-dependent manner in
HL-60 cells. Que inhibited HL-60 cell proliferation. The values of IC50 and 95%
confidence limits were 43 (30-61) mumol.L-1 after 48-h treatment with Que.
CONCLUSION: Que induced apoptosis in HL-60 cells.
Keywords:
HL-60 cells.
METHODS: DNA fragmentation was visualized by agarose gel electrophoresis.
Inhibition of proliferation was measured with a colorimetric MTT-assay. The DNA
degradation was determined using flow cytometry, and the microscopic changes were
observed by an electron microscope.
RESULTS: Que 15-120 mumol.L-1 elicited typical apoptosis morphological changes
including condensed chromatin, nuclear fragmentation, and reduction in volume.
DNA fragmentation and DNA degradation in a concentration-dependent manner in
HL-60 cells. Que inhibited HL-60 cell proliferation. The values of IC50 and 95%
confidence limits were 43 (30-61) mumol.L-1 after 48-h treatment with Que.
CONCLUSION: Que induced apoptosis in HL-60 cells.