Effect of tripterine on collagen-induced arthritis in rats
Abstract
AIM: To study the therapeutic effect of tripterine (Tri) on collagen-induced
arthritis (CIA).
METHODS: Collagen type II (Col) 1.5 mg was injected intradermally to induce CIA
in rats. Hind paw volumes of rats were measured with a water displacement method.
The serum anti-collagen antibody was measured by an enzyme-linked immunosorbent
assay. Delayed hypersensitivity was reflected by skin response to Col.
Interleukin-1 (IL-1) and interleukin-2 (IL-2) activities were evaluated by
[3H]TdR uptake. Joint was evaluated histologically.
RESULTS: Tri 15 and 30 mg.kg-1.d-1 given i.g. to rats 3 d after the first sign of
arthritis reduced inflammatory swelling, suppressed humoral and skin response to
Col, inhibited IL-2 and IL-1 production, reduced pathological progression of
joint.
CONCLUSION: Tri has a therapeutic effect on CIA.
Keywords:
arthritis (CIA).
METHODS: Collagen type II (Col) 1.5 mg was injected intradermally to induce CIA
in rats. Hind paw volumes of rats were measured with a water displacement method.
The serum anti-collagen antibody was measured by an enzyme-linked immunosorbent
assay. Delayed hypersensitivity was reflected by skin response to Col.
Interleukin-1 (IL-1) and interleukin-2 (IL-2) activities were evaluated by
[3H]TdR uptake. Joint was evaluated histologically.
RESULTS: Tri 15 and 30 mg.kg-1.d-1 given i.g. to rats 3 d after the first sign of
arthritis reduced inflammatory swelling, suppressed humoral and skin response to
Col, inhibited IL-2 and IL-1 production, reduced pathological progression of
joint.
CONCLUSION: Tri has a therapeutic effect on CIA.