Effect of saponins of Panax notoginseng on synaptosomal 45Ca uptake.
Abstract
AIM: To explore the calcium uptake antagonism of saponins of Panax notoginseng
(PNS).
METHODS: Synaptosomes were prepared from rat cerebral cortex by using
differential Ficoll gradients. The effects of PNS on synaptosomal 45Ca uptake
were measured in vitro or after acute treatment.
RESULTS: PNS 50-800 mg.L-1 produced a concentration-rated inhibition of Ca2+
uptake [IC50 = 111 (46-176) mg.L-1]. Both initial and maximal uptake were
inhibited. Similar effect was obtained after acute PNS treatment with 200 mg.kg-1
i.p. The blocking effect of PNS was reversed by calcium in media.
CONCLUSION: PNS is a calcium channel blocker in neurons.
Keywords:
(PNS).
METHODS: Synaptosomes were prepared from rat cerebral cortex by using
differential Ficoll gradients. The effects of PNS on synaptosomal 45Ca uptake
were measured in vitro or after acute treatment.
RESULTS: PNS 50-800 mg.L-1 produced a concentration-rated inhibition of Ca2+
uptake [IC50 = 111 (46-176) mg.L-1]. Both initial and maximal uptake were
inhibited. Similar effect was obtained after acute PNS treatment with 200 mg.kg-1
i.p. The blocking effect of PNS was reversed by calcium in media.
CONCLUSION: PNS is a calcium channel blocker in neurons.