Protection of ebselen against anoxic damage of cultured neurons of cerebral cortex
Abstract
AIM: To study the protective effect of ebselen on anoxic damage of brain cells.
METHODS: On d 10 after plating of the cortical neurons from 1-d-old rat, cultures
were placed under 95% N2 + 5% CO2 for 2-6 h. Lactate dehydrogenase (LDH) in
supernatant, thiobarbituric acid reactive substance (TBARS) and glutathione
peroxidase (GSH-Px) activity of neurons were determined.
RESULTS: Under anoxia, efflux of LDH and TBARS from cultured neurons increased
while GSH-Px activity decreased. Ebselen reduced the efflux of LDH and TBARS in a
dose-related manner and increased the total GSH-Px activity.
CONCLUSION: Ebselen can protect neurons from anoxic damage.
Keywords:
METHODS: On d 10 after plating of the cortical neurons from 1-d-old rat, cultures
were placed under 95% N2 + 5% CO2 for 2-6 h. Lactate dehydrogenase (LDH) in
supernatant, thiobarbituric acid reactive substance (TBARS) and glutathione
peroxidase (GSH-Px) activity of neurons were determined.
RESULTS: Under anoxia, efflux of LDH and TBARS from cultured neurons increased
while GSH-Px activity decreased. Ebselen reduced the efflux of LDH and TBARS in a
dose-related manner and increased the total GSH-Px activity.
CONCLUSION: Ebselen can protect neurons from anoxic damage.