Inhibitory effect of artemether on proteinase of Schistosoma japonicum.
Abstract
AIM: To study the effect of artemether (Art) on the thio proteinase
(""hemoglobinase"", Hem) of Schistosoma japonicum.
METHODS: Hem was extracted from S japonicum adults. The inhibitory effect of Art
on the activity of Hem to degrade human hemoglobin (Hgb) was examined with
UV-photometer at 280 nm, SDS-PAGE and scanning at 600 nm on a chromoscanner.
RESULTS: Human Hgb was degraded at pH 4.0 by the Hem. The activities of Hem
preincubated at 37 degrees C with Art 0.14, 1.4, and 14 mmol.L-1, were inhibited
by 30.2%, 39.8%, and 45.0%, respectively.
CONCLUSION: Art possesses an inhibitory effect to Hem of S japonicum.
Keywords:
(""hemoglobinase"", Hem) of Schistosoma japonicum.
METHODS: Hem was extracted from S japonicum adults. The inhibitory effect of Art
on the activity of Hem to degrade human hemoglobin (Hgb) was examined with
UV-photometer at 280 nm, SDS-PAGE and scanning at 600 nm on a chromoscanner.
RESULTS: Human Hgb was degraded at pH 4.0 by the Hem. The activities of Hem
preincubated at 37 degrees C with Art 0.14, 1.4, and 14 mmol.L-1, were inhibited
by 30.2%, 39.8%, and 45.0%, respectively.
CONCLUSION: Art possesses an inhibitory effect to Hem of S japonicum.