Effects of 16 drugs on immunological liver injury induced by BCG + lipopolysaccharides in mice
Abstract
AIM: To evaluate anti-hepatitis drugs.
METHODS: Mice were injected i.v. with viable BCG 5 x 10(7) (live) bacilli/mouse,
after 10 d, i.v. lipopolysaccharides (LPS) 5-10 micrograms/mouse. Serum
aminotransferase levels and liver tissue were examined 10 h after i.v. LPS.
Sixteen drugs were evaluated in this model.
RESULTS: The level of AlaAT and AspAT were increased markedly. Submassive
necrosis and infiltrations of granulocytes and lymphocytes were seen, which were
not recovered till 14 d after i.v. LPS. Administration of interferon alpha-2a,
Ara-AMP, hepatocyte growth factor, biphenyl dimethyl dicarboxylate, bicyclol,
prednisolone, and cyclophosphamide for 10 d prior to i.v. LPS suppressed the
elevation of serum AlaAT and AspAT.
CONCLUSION: The model of immunological liver injury reproduced by i.v. BCG + LPS
in mice may be used for evaluating anti-hepatitis drugs.
Keywords:
METHODS: Mice were injected i.v. with viable BCG 5 x 10(7) (live) bacilli/mouse,
after 10 d, i.v. lipopolysaccharides (LPS) 5-10 micrograms/mouse. Serum
aminotransferase levels and liver tissue were examined 10 h after i.v. LPS.
Sixteen drugs were evaluated in this model.
RESULTS: The level of AlaAT and AspAT were increased markedly. Submassive
necrosis and infiltrations of granulocytes and lymphocytes were seen, which were
not recovered till 14 d after i.v. LPS. Administration of interferon alpha-2a,
Ara-AMP, hepatocyte growth factor, biphenyl dimethyl dicarboxylate, bicyclol,
prednisolone, and cyclophosphamide for 10 d prior to i.v. LPS suppressed the
elevation of serum AlaAT and AspAT.
CONCLUSION: The model of immunological liver injury reproduced by i.v. BCG + LPS
in mice may be used for evaluating anti-hepatitis drugs.