alpha-Anordrin-induced apoptosis of leukemia K562 cells is not prevented by cicloheximide
Abstract
AIM: To study effect of protein synthesis inhibitor cicloheximide (Cic) on the
apoptosis induced by alpha-anordrin (Ano) in leukemia K562 cells.
METHODS: Morphological changes were observed by fluorescent microscopy. DNA
content was measured by flow cytometry. DNA fragmentation was analyzed by agarose
gel electrophoresis.
RESULTS: Exposure of K562 cells to Ano 50 mumol.L-1 for 24 h induced apoptotic
cell death. Cic 1 mumol.L-1 did not abrogate or delay this effect. Indeed,
Ano-induced apoptosis was augmented by Cic. Cic 100 mumol.L-1 itself stimulated
25% K562 cell apoptosis after 24-h culture.
CONCLUSION: Ano-induced apoptosis was independent of de novo protein synthesis.
Keywords:
apoptosis induced by alpha-anordrin (Ano) in leukemia K562 cells.
METHODS: Morphological changes were observed by fluorescent microscopy. DNA
content was measured by flow cytometry. DNA fragmentation was analyzed by agarose
gel electrophoresis.
RESULTS: Exposure of K562 cells to Ano 50 mumol.L-1 for 24 h induced apoptotic
cell death. Cic 1 mumol.L-1 did not abrogate or delay this effect. Indeed,
Ano-induced apoptosis was augmented by Cic. Cic 100 mumol.L-1 itself stimulated
25% K562 cell apoptosis after 24-h culture.
CONCLUSION: Ano-induced apoptosis was independent of de novo protein synthesis.