Effects of dexamethasone and ibuprofen on LPS-induced gene expression of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung
Abstract
AIM: To study the kinetics of tumor necrosis factor alpha (TNF alpha),
interleukine-1 (IL-1 beta), and macrophage inflammatory protein-1 alpha (MIP-1
alpha) gene expression in rat lung after i.p. lipopolysaccharides (LPS) and the
effect of dexamethasone (Dex) and ibuprofen (Ibu) on the cytokines gene
expression.
METHODS: The amount of Evans blue in lung was measured by fluorescence method.
The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung were
assessed by slot blot analysis.
RESULTS: The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung
after i.p. LPS increased in a dose-dependent manner, and peaked at 2, 6, and 12
h, respectively. Both Dex 50 mg.kg-1 and Ibu 90 mg.kg-1 injected at 1 h before
i.p. LPS markedly decreased the content of Evans blue in lung at 1 h after i.p.
LPS. After Dex or Ibu pretreatment, the peak levels of TNF alpha, IL-1 beta, and
MIP-1 alpha mRNA decreased markedly compared with LPS alone.
CONCLUSION: The gene expression of TNF alpha, IL-1 beta, and MIP-1 alpha in rat
lung increased after i.p. LPS. Dex and Ibu prevented LPS-induced lung injury
through inhibiting the cytokines gene expression.
Keywords:
interleukine-1 (IL-1 beta), and macrophage inflammatory protein-1 alpha (MIP-1
alpha) gene expression in rat lung after i.p. lipopolysaccharides (LPS) and the
effect of dexamethasone (Dex) and ibuprofen (Ibu) on the cytokines gene
expression.
METHODS: The amount of Evans blue in lung was measured by fluorescence method.
The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung were
assessed by slot blot analysis.
RESULTS: The mRNA levels of TNF alpha, IL-1 beta, and MIP-1 alpha in rat lung
after i.p. LPS increased in a dose-dependent manner, and peaked at 2, 6, and 12
h, respectively. Both Dex 50 mg.kg-1 and Ibu 90 mg.kg-1 injected at 1 h before
i.p. LPS markedly decreased the content of Evans blue in lung at 1 h after i.p.
LPS. After Dex or Ibu pretreatment, the peak levels of TNF alpha, IL-1 beta, and
MIP-1 alpha mRNA decreased markedly compared with LPS alone.
CONCLUSION: The gene expression of TNF alpha, IL-1 beta, and MIP-1 alpha in rat
lung increased after i.p. LPS. Dex and Ibu prevented LPS-induced lung injury
through inhibiting the cytokines gene expression.