Inhibitory effects of nifedipine on DNA and protein synthesis in cultured cardiac nonmyocytes of neonatal rats
Abstract
AIM: To observe the inhibitory effects of nifedipine (Nif) on cardiac nonmyocytes
growth and proliferation.
METHODS: Using nonmyocytes in culture as a model, [3H]thymidine and [3H]leucine
incorporation were measured.
RESULTS: There was a significant decrease in cell number and in total cellular
protein after 72-h exposure to Nif 1 mumol.L-1 in the presence of angiotensin II
(Ang II). A 48-h exposure to 1, 10, 100, 1000 nmol.L-1 of Ang II caused a 19%,
35%, 46%, 48% increase in protein synthesis and 27%, 46%, 56%, 57% increase in
DNA synthesis. Nif 1, 3, and 10 mumol.L-1 were able to reduce the Ang II 100
nmol.L-1-induced increase of protein synthesis and DNA synthesis.
CONCLUSION: Nif had a direct inhibitory action on the growth of nonmyocytes,
which was related to the regression of cardical hypertrophy.
Keywords:
growth and proliferation.
METHODS: Using nonmyocytes in culture as a model, [3H]thymidine and [3H]leucine
incorporation were measured.
RESULTS: There was a significant decrease in cell number and in total cellular
protein after 72-h exposure to Nif 1 mumol.L-1 in the presence of angiotensin II
(Ang II). A 48-h exposure to 1, 10, 100, 1000 nmol.L-1 of Ang II caused a 19%,
35%, 46%, 48% increase in protein synthesis and 27%, 46%, 56%, 57% increase in
DNA synthesis. Nif 1, 3, and 10 mumol.L-1 were able to reduce the Ang II 100
nmol.L-1-induced increase of protein synthesis and DNA synthesis.
CONCLUSION: Nif had a direct inhibitory action on the growth of nonmyocytes,
which was related to the regression of cardical hypertrophy.