Tumor necrosis factor mediated release of platelet-derived growth factor from bovine cerebral microvascular endothelial cells
Abstract
AIM: To study the effect of tumor necrosis factor (TNF) on platelet-derived
growth factor (PDGF) release from bovine cerebral microvascular endothelial cells
(BCMEC), and the protection of drugs on proliferation of bovine cerebral
microvascular smooth muscle cells (BCMSMC).
METHODS: BCMEC and BCMSMC were cultured in vitro. Effects of TNF on PDGF release
from BCMEC were investigated by crystal violet assay.
RESULTS: TNF did not promote the proliferation of BCMSMC directly. But the
postculture medium of BCMEC stimulated by TNF promoted the proliferation of
BCMSMC in a concentration (5-20 micrograms.L-1)-dependent manner, and the
mitogenic activity was neutralized by antibody to PDGF. The BCMSMC proliferation
was increased 34 +/- 4% over control by TNF (20 micrograms.L-1). Imperatorin
(Imp), iso-imperatorin (iso-Imp), and 6-(alpha, alpha-phenylacetylpiperazinyl)
phenyl-5-methyl-4, 5-dihydro-3 (2H)-pyridazinone (PMDP) 1-100 mumol.L-1 did not
affect the PDGF release from BCMEC stimulated by TNF, but inhibited the BCMSMC
proliferation promoted by PDGF concentration-dependently.
CONCLUSION: TNF promoted PDGF release from BCMEC. The proliferation of BCMSMC
stimulated by TNF must be mediated by growth factor, such as PDGF. Imp, iso-Imp,
PMDP inhibited proliferation of BCMSMC stimulated by PDGF.
Keywords:
growth factor (PDGF) release from bovine cerebral microvascular endothelial cells
(BCMEC), and the protection of drugs on proliferation of bovine cerebral
microvascular smooth muscle cells (BCMSMC).
METHODS: BCMEC and BCMSMC were cultured in vitro. Effects of TNF on PDGF release
from BCMEC were investigated by crystal violet assay.
RESULTS: TNF did not promote the proliferation of BCMSMC directly. But the
postculture medium of BCMEC stimulated by TNF promoted the proliferation of
BCMSMC in a concentration (5-20 micrograms.L-1)-dependent manner, and the
mitogenic activity was neutralized by antibody to PDGF. The BCMSMC proliferation
was increased 34 +/- 4% over control by TNF (20 micrograms.L-1). Imperatorin
(Imp), iso-imperatorin (iso-Imp), and 6-(alpha, alpha-phenylacetylpiperazinyl)
phenyl-5-methyl-4, 5-dihydro-3 (2H)-pyridazinone (PMDP) 1-100 mumol.L-1 did not
affect the PDGF release from BCMEC stimulated by TNF, but inhibited the BCMSMC
proliferation promoted by PDGF concentration-dependently.
CONCLUSION: TNF promoted PDGF release from BCMEC. The proliferation of BCMSMC
stimulated by TNF must be mediated by growth factor, such as PDGF. Imp, iso-Imp,
PMDP inhibited proliferation of BCMSMC stimulated by PDGF.